Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets

Abstract: Inflammatory intestinal diseases are characterized by abnormal immune responses and affect distinct locations of the gastrointestinal tract. Although the role of several immune subsets in driving intestinal pathology has been studied, a system-wide approach that simultaneously interrogates all major lineages on a single-cell basis is lacking. We used high-dimensional mass cytometry to generate a system-wide view of the human mucosal immune system in health and disease. We distinguished 142 immune subsets and through computational applications found distinct immune subsets in peripheral blood mononuclear cells and intestinal biopsies that distinguished patients from controls. In addition, mucosal lymphoid malignancies were readily detected as well as precursors from which these likely derived. These findings indicate that an integrated high-dimensional analysis of the entire immune system can identify immune subsets associated with the pathogenesis of complex intestinal disorders. This might have implications for diagnostic procedures, immune-monitoring, and treatment of intestinal diseases and mucosal malignancies.

 LUMC Best Article Prize 2016 (non clinical)
Vincent van Unen, Na Li, Ilse Molendijk, Mine Temurhan, Thomas Höllt, et al. Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets. Immunity, 44(5): pp. 1227–1239, 2016.
@article { bib:2016_immunity_vvu,
author = { Vincent van Unen and Na Li and Ilse Molendijk and Mine Temurhan and Thomas H{\"o}llt and Andrea E. van der Meulen-de Jong and Hein W. Verspaget and M. Luisa Mearin and Chris J. Mulder and Jeroen van Bergen and Boudewijn Lelieveldt and Frits Koning },
title = { Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets },
journal = { Immunity },
volume = { 44 },
number = { 5 },
pages = { 1227 -- 1239 },
year = { 2016 },
doi = { 10.1016/j.immuni.2016.04.014 },
}