Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.

Andrea Cossarizza, Hyun‐Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49(10): pp. 1457–1973, 2019.
@article { bib:2019_cytometry_guidelines,
author = { Andrea Cossarizza and Hyun‐Dong Chang and Andreas Radbruch and Andreas Acs and Dieter Adam and Sabine Adam‐Klages and William W. Agace and Nima Aghaeepour and M{\"u}beccel Akdis and Matthieu Allez and Larissa Nogueira Almeida and Giorgia Alvisi and Graham Anderson and Immanuel Andr{\"a} and Francesco Annunziato and Achille Anselmo and Petra Bacher and Cosima T. Baldari and Sudipto Bari and Vincenzo Barnaba and Joana Barros‐Martins and Luca Battistini and Wolfgang Bauer and Sabine Baumgart and Nicole Baumgarth and Dirk Baumjohann and Bianka Baying and Mary Bebawy and Burkhard Becher and Wolfgang Beisker and Vladimir Benes and Rudi Beyaert and Alfonso Blanco and Dominic A. Boardman and Christian Bogdan and Jessica G. Borger and Giovanna Borsellino and Philip E. Boulais and Jolene A. Bradford and Dirk Brenner and Ryan R. Brinkman and Anna E. S. Brooks and Dirk H. Busch and Martin B{\"u}scher and Timothy P. Bushnell and Federica Calzetti and Garth Cameron and Ilenia Cammarata and Xuetao Cao and Susanna L. Cardell and Stefano Casola and Marco A. Cassatella and Andrea Cavani and Antonio Celada and Lucienne Chatenoud and Pratip K. Chattopadhyay and Sue Chow and Eleni Christakou and Luka Čičin‐Šain and Mario Clerici and Federico S. Colombo and Laura Cook and Anne Cooke and Andrea M. Cooper and Alexandra J. Corbett and Antonio Cosma and Lorenzo Cosmi and Pierre G. Coulie and Ana Cumano and Ljiljana Cvetkovic and Van Duc Dang and Chantip Dang‐Heine and Martin S. Davey and Derek Davies and Sara De Biasi and Genny Del Zotto and Gelo Victoriano Dela Cruz and Michael Delacher and Silvia Della Bella and Paolo Dellabona and G{\"u}nnur Deniz and Mark Dessing and James P. Di Santo and Andreas Diefenbach and Francesco Dieli and Andreas Dolf and Thomas D{\"o}rner and Regine J. Dress and Diana Dudziak and Michael Dustin and Charles‐Antoine Dutertre and Friederike Ebner and Sidonia B. G. Eckle and Matthias Edinger and Pascale Eede and G{\"o}tz R.A. Ehrhardt and Marcus Eich and Pablo Engel and Britta Engelhardt and Anna Erdei and Charlotte Esser and Bart Everts and Maximilien Evrard and Christine S. Falk and Todd A. Fehniger and Mar Felipo‐Benavent and Helen Ferry and Markus Feuerer and Andrew Filby and Kata Filkor and Simon Fillatreau and Marie Follo and Irmgard F{\"o}rster and John Foster and Gemma A. Foulds and Britta Frehse and Paul S. Frenette and Stefan Frischbutter and Wolfgang Fritzsche and David W. Galbraith and Anastasia Gangaev and Natalio Garbi and Brice Gaudilliere and Ricardo T. Gazzinelli and Jens Geginat and Wilhelm Gerner and Nicholas A. Gherardin and Kamran Ghoreschi and Lara Gibellini and Florent Ginhoux and Keisuke Goda and Dale I. Godfrey and Christoph Goettlinger and Jose M. Gonz{\lez‐Navajas and Carl S. Goodyear and Andrea Gori and Jane L. Grogan and Daryl Grummitt and Andreas Gr{\"u}tzkau and Claudia Haftmann and Jonas Hahn and Hamida Hammad and G{\"u}nter H{\"a}mmerling and Leo Hansmann and Goran Hansson and Christopher M. Harpur and Susanne Hartmann and Andrea Hauser and Anja E. Hauser and David L. Haviland and David Hedley and Daniela C. Hern{\ndez and Guadalupe Herrera and Martin Herrmann and Christoph Hess and Thomas H{\"o}fer and Petra Hoffmann and Kristin Hogquist and Tristan Holland and Thomas H{\"o}llt and Rikard Holmdahl and Pleun Hombrink and Jessica P. Houston and Bimba F. Hoyer and Bo Huang and Fang‐Ping Huang and Johanna E. Huber and Jochen Huehn and Michael Hundemer and Christopher A. Hunter and William Y. K. Hwang and Anna Iannone and Florian Ingelfinger and Sabine M Ivison and Hans‐Martin J{\"a}ck and Peter K. Jani and Beatriz J{\vega and Stipan Jonjic and Toralf Kaiser and Tomas Kalina and Thomas Kamradt and Stefan H. E. Kaufmann and Baerbel Keller and Steven L. C. Ketelaars and Ahad Khalilnezhad and Srijit Khan and Jan Kisielow and Paul Klenerman and Jasmin Knopf and Hui‐Fern Koay and Katja Kobow and Jay K. Kolls and Wan Ting Kong and Manfred Kopf and Thomas Korn and Katharina Kriegsmann and Hendy Kristyanto and Thomas Kroneis and Andreas Krueger and Jenny K{\"u}hne and Christian Kukat and D{\sir{\e Kunkel and Heike Kunze‐Schumacher and Tomohiro Kurosaki and Christian Kurts and Pia Kvistborg and Immanuel Kwok and Jonathan Landry and Olivier Lantz and Paola Lanuti and Francesca LaRosa and Agn{Agne}s Lehuen and Salom{\ LeibundGut‐Landmann and Michael D. Leipold and Leslie Y.T. Leung and Megan K. Levings and Andreia C. Lino and Francesco Liotta and Virginia Litwin and Yanling Liu and Hans‐Gustaf Ljunggren and Michael Lohoff and Giovanna Lombardi and Lilly Lopez and Miguel L{\pez‐Botet and Amy E. Lovett‐Racke and Erik Lubberts and Herve Luche and Burkhard Ludewig and Enrico Lugli and Sebastian Lunemann and Holden T. Maecker and Laura Maggi and Orla Maguire and Florian Mair and Kerstin H. Mair and Alberto Mantovani and Rudolf A. Manz and Aaron J. Marshall and Alicia Mart{\nez‐Romero and Gl{Glo}ria Martrus and Ivana Marventano and Wlodzimierz Maslinski and Giuseppe Matarese and Anna Vittoria Mattioli and Christian Mauer{\"o}der and Alessio Mazzoni and James McCluskey and Mairi McGrath and Helen M. McGuire and Iain B. McInnes and Henrik E. Mei and Fritz Melchers and Susanne Melzer and Dirk Mielenz and Stephen D. Miller and Kingston H.G. Mills and Hans Minderman and Jenny Mj{\"o}sberg and Jonni Moore and Barry Moran and Lorenzo Moretta and Tim R. Mosmann and Susann M{\"u}ller and Gabriele Multhoff and Luis Enrique Mu{\~n}oz and Christian M{\"u}nz and Toshinori Nakayama and Milena Nasi and Katrin Neumann and Lai Guan Ng and Antonia Niedobitek and Sussan Nourshargh and Gabriel N{\{\~n}ez and Jos{\‐Enrique O'Connor and Aaron Ochel and Anna Oja and Diana Ordonez and Alberto Orfao and Eva Orlowski‐Oliver and Wenjun Ouyang and Annette Oxenius and Raghavendra Palankar and Isabel Panse and Kovit Pattanapanyasat and Malte Paulsen and Dinko Pavlinic and Livius Penter and P{\"a}rt Peterson and Christian Peth and Jordi Petriz and Federica Piancone and Winfried F. Pickl and Silvia Piconese and Marcello Pinti and A. Graham Pockley and Malgorzata Justyna Podolska and Zhiyong Poon and Katharina Pracht and Immo Prinz and Carlo E. M. Pucillo and Sally A. Quataert and Linda Quatrini and Kylie M. Quinn and Helena Radbruch and Tim R. D. J. Radstake and Susann Rahmig and Hans‐Peter Rahn and Bartek Rajwa and Gevitha Ravichandran and Yotam Raz and Jonathan A. Rebhahn and Diether Recktenwald and Dorothea Reimer and Caetano Reis e Sousa and Ester B.M. Remmerswaal and Lisa Richter and Laura G. Rico and Andy Riddell and Aja M. Rieger and J. Paul Robinson and Chiara Romagnani and Anna Rubartelli and J{\"u}rgen Ruland and Armin Saalm{\"u}ller and Yvan Saeys and Takashi Saito and Shimon Sakaguchi and Francisco Sala‐de‐Oyanguren and Yvonne Samstag and Sharon Sanderson and Inga Sandrock and Angela Santoni and Ramon Bellm{Ramon Bellma}s Sanz and Marina Saresella and Catherine Sautes‐Fridman and Birgit Sawitzki and Linda Schadt and Alexander Scheffold and Hans U. Scherer and Matthias Schiemann and Frank A. Schildberg and Esther Schimisky and Andreas Schlitzer and Josephine Schlosser and Stephan Schmid and Steffen Schmitt and Kilian Schober and Daniel Schraivogel and Wolfgang Schuh and Thomas Sch{\"u}ler and Reiner Schulte and Axel Ronald Schulz and Sebastian R. Schulz and Cristiano Scott{\ and Daniel Scott‐Algara and David P. Sester and T. Vincent Shankey and Bruno Silva‐Santos and Anna Katharina Simon and Katarzyna M. Sitnik and Silvano Sozzani and Daniel E. Speiser and Josef Spidlen and Anders Stahlberg and Alan M. Stall and Natalie Stanley and Regina Stark and Christina Stehle and Tobit Steinmetz and Hannes Stockinger and Yousuke Takahama and Kiyoshi Takeda and Leonard Tan and Attila T{\rnok and Gisa Tiegs and Gergely Toldi and Julia Tornack and Elisabetta Traggiai and Mohamed Trebak and Timothy I.M. Tree and Joe Trotter and John Trowsdale and Maria Tsoumakidou and Henning Ulrich and Sophia Urbanczyk and Willem van de Veen and Maries van den Broek and Edwin van der Pol and Sofie Van Gassen and Gert Van Isterdael and Ren{\ A.W. van Lier and Marc Veldhoen and Salvador Vento‐Asturias and Paulo Vieira and David Voehringer and Hans‐Dieter Volk and Anouk von Borstel and Konrad von Volkmann and Ari Waisman and Rachael V. Walker and Paul K. Wallace and Sa A. Wang and Xin M. Wang and Michael D. Ward and Kirsten A Ward‐Hartstonge and Klaus Warnatz and Gary Warnes and Sarah Warth and Claudia Waskow and James V. Watson and Carsten Watzl and Leonie Wegener and Thomas Weisenburger and Annika Wiedemann and J{\"u}rgen Wienands and Anneke Wilharm and Robert John Wilkinson and Gerald Willimsky and James B. Wing and Rieke Winkelmann and Thomas H. Winkler and Oliver F. Wirz and Alicia Wong and Peter Wurst and Jennie H. M. Yang and Juhao Yang and Maria Yazdanbakhsh and Liping Yu and Alice Yue and Hanlin Zhang and Yi Zhao and Susanne Maria Ziegler and Christina Zielinski and Jakob Zimmermann and Arturo Zychlinsky },
title = { Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) },
journal = { European Journal of Immunology },
volume = { 49 },
number = { 10 },
pages = { 1457 -- 1973 },
year = { 2019 },
doi = { 10.1002/eji.201970107 },
}
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